White muscle lactate and pyruvate concentrations in rested flounder, Platichthys flesus, and plaice, Pleuronectes platessa: a re-evaluation of sampling techniques.

Adcock, P. and Dando, P. R. 1983 White muscle lactate and pyruvate concentrations in rested flounder, Platichthys flesus, and plaice, Pleuronectes platessa: a re-evaluation of sampling techniques.. Journal of the Marine Biological Association of the United Kingdom, 63 (4). 897-903. https://doi.org/10.1017/S0025315400071307

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Abstract/Summary

Rapid fixing of skeletal muscle by a ‘freeze-clamp’ technique results in up to a 3-fold lower lactate, a slightly higher pyruvate concentration and a 2- to 4-fold decrease in lactate/pyruvate ratio, to the lowest value yet recorded for fish muscle, when compared with the more usual method of direct immersion in liquid nitrogen. This is attributed to the faster cooling rate of freeze-clamped muscle minimizing ‘sampling anoxia’. Immobilizing fish either by anaesthetic or stunning produces no significant change in metabolite levels. It is concluded that it is relatively easy to handle quiescent flatfish, but light anaesthesia ensures no muscular activity.

Item Type:	Publication - Article
Subjects:	Marine Sciences
Divisions:	Marine Biological Association of the UK > Other (MBA)
Depositing User:	Professor Paul Dando
Date made live:	09 Aug 2022 14:38
Last Modified:	09 Aug 2022 14:38
URI:	https://plymsea.ac.uk/id/eprint/9761

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