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Schiano di Visconte, G, Allen, MJ and Spicer, A 2021 Novel Capsular Polysaccharide from Lobochlamys segnis. Polysaccharides, 2 (1). 121-137. https://doi.org/10.3390/polysaccharides2010009
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Abstract/Summary
In recent years there has been a significant effort from food, nutraceutical, cosmeceutical, pharmaceutical, and biomedical industries to discover and develop new natural ingredients. Microalgae have been recognised as potential sources of high value chemicals, with most attention focused upon antioxidants, pigments, and specialty oils. An under-exploited group of biochemicals produced by microalgae are extracellular polymeric substances (EPS). Lobochlamys segnis (formerly called Chlamydomonas segnis) was previously reported to produce a large amount of capsular polysaccharide (CPS) closely related to hyaluronan (HA) under stress conditions. In this work, the purified CPS was characterised and shown to have an average molecular mass (Mn) of about 3.7 MDa, and displayed a highly branched random coil structure unlike the simple repeating linear HA polysaccharide. Chemical analysis showed the presence of galactose, glucuronic acid, and glucose sugars confirming that the product has a different composition to that of HA. Mixotrophic growth and stress conditions were identified and improved upon with a pH control system using acetic acid solution to induce efficient production of CPS. Extraction and purification conditions were also identified exploiting the high Mn of the product. The CPS showed thickening properties and both significant antioxidant capacity and reducing power, which could have commercial applications. This is the first report on the characterization of this novel polysaccharide with non-Newtonian properties similar to HA.
| Item Type: | Publication - Article |
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| Additional Keywords: | microalgae; biotechnology; EPS; hyaluronan |
| Divisions: | Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations |
| Depositing User: | S Hawkins |
| Date made live: | 14 May 2021 08:40 |
| Last Modified: | 09 Jan 2023 10:13 |
| URI: | https://plymsea.ac.uk/id/eprint/9218 |
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