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Smyth, TJ, Tarran, GA and Sathyendranath, S 2020 Sub-micron picoplankton shape, orientation and internal structure combine to preferentially amplify forward scatter. Optics Express. https://doi.org/10.1364/OE.413576 (In Press)
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Abstract/Summary
Compelling evidence is presented that sub-micron picoplankton shape, internal structure and orientation in combination leads to a disproportionate enhancement of differential forward scatter compared with differential side scatter when analyzed with a flow cytometer. Theoretical evidence is provided which results in an order of magnitude amplification in the forward scatter direction, with little or no change in the side scatter: this discounts the possibility of “doublets” caused by multiple particles simultaneously present in the laser beam. Observational evidence from progressively finer filtered seawater samples shows up to three orders of magnitude enhancement in the forward scatter direction and sizes of Prochlorococcus close to that reported in the literature (0.61±0.17µm). It therefore seems likely that flow cytometrically observed “bi-modal size distributions” of Prochlorococcus are instead the manifestation of intra-population differences in shape (spherical – prolate with preferential alignment) and internal structure (homogenous – heterogenous). ©
| Item Type: | Publication - Article |
|---|---|
| Divisions: | Plymouth Marine Laboratory > National Capability categories > Single Centre NC - CLASS (expired) Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations |
| Depositing User: | S Hawkins |
| Date made live: | 04 Jan 2021 17:43 |
| Last Modified: | 04 Jan 2021 17:43 |
| URI: | https://plymsea.ac.uk/id/eprint/9096 |
Available Versions of this Item
- Sub-micron picoplankton shape, orientation and internal structure combine to preferentially amplify forward scatter. (deposited 04 Jan 2021 17:43) [Currently Displayed]
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