Tools
Tools
Warwick-Dugdale, J, Solonenko, N, Moore, K, Chittick, L, Gregory, AC, Allen, MJ, Sullivan, MB and Temperton, B 2019 Long-read viral metagenomics captures abundant and microdiverse viral populations and their niche-defining genomic islands. PeerJ, 7. e6800. https://doi.org/10.7717/peerj.6800
|
Text
peerj-6800_publishedL4paper.pdf - Published Version Available under License Creative Commons Attribution. Download (4MB) | Preview |
Abstract/Summary
Marine viruses impact global biogeochemical cycles via their influence on host community structure and function, yet our understanding of viral ecology is constrained by limitations in host culturing and a lack of reference genomes and ‘universal’ gene markers to facilitate community surveys. Short-read viral metagenomic studies have provided clues to viral function and first estimates of global viral gene abundance and distribution, but their assemblies are confounded by populations with high levels of strain evenness and nucleotide diversity (microdiversity), limiting assembly of some of the most abundant viruses on Earth. Such features also challenge assembly across genomic islands containing niche-defining genes that drive ecological speciation. These populations and features may be successfully captured by single-virus genomics and fosmid-based approaches, at least in abundant taxa, but at considerable cost and technical expertise. Here we established a low-cost, low-input, high throughput alternative sequencing and informatics workflow to improve viral metagenomic assemblies using short-read and long-read technology. The ‘VirION’ (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. We then then applied VirION to the first metagenome from a natural viral community from the Western English Channel. In comparison to a short-read only approach, VirION: (i) increased number and completeness of assembled viral genomes; (ii) captured abundant, highly microdiverse virus populations, and (iii) captured more and longer genomic islands. Together, these findings suggest that VirION provides a high throughput and cost-effective alternative to fosmid and single-virus genomic approaches to more comprehensively explore viral communities in nature.
| Item Type: | Publication - Article |
|---|---|
| Subjects: | Ecology and Environment Marine Sciences Oceanography |
| Divisions: | Plymouth Marine Laboratory > National Capability categories > Western Channel Observatory Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations Plymouth Marine Laboratory > Science Areas > Marine Ecology and Biodiversity |
| Depositing User: | Kim Hockley |
| Date made live: | 09 May 2019 14:30 |
| Last Modified: | 25 Apr 2020 10:00 |
| URI: | https://plymsea.ac.uk/id/eprint/8188 |
Actions (login required)
 |
View Item |