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Abeln, F, Fan, J, Budarin, VL, Briers, H, Parsons, S, Allen, MJ, Henk, DA, Clark, J and Chuck, CJ 2019 Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima. Algal Research, 38. 101411. https://doi.org/10.1016/j.algal.2019.101411
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Abstract/Summary
Macroalgae (seaweeds) represent an emerging resource for food and the production of commodity and specialty chemicals. In this study, a single-step microwave process was used to depolymerise a range of macroalgae native to the United Kingdom, producing a growth medium suitable for microbial fermentation. The medium contained a range of mono- and polysaccharides as well as macro- and micronutrients that could be metabolised by the oleaginous yeast Metschnikowia pulcherrima. Among twelve macroalgae species, the brown seaweeds exhibited the highest fermentation potential, especially the kelp Saccharina latissima. Applying a portfolio of ten native M. pulcherrima strains, yeast growth kinetics, as well as production of lipids and 2-phenylethanol were examined, with productivity and growth rate being strain dependent. On the 2 L scale, 6.9 g L−1 yeast biomass – a yield of 0.14 g g−1 with respect to the supplied macroalgae – containing 37.2% (w/w) lipid was achieved through utilisation of the proteins, mono- and polysaccharides from S. latissima, with no additional enzymes. In addition, the yeast degraded a range of fermentation inhibitors released upon microwave processing at high temperatures and long holding times. As macroalgae can be cultured to food grade, this system offers a novel, potentially low-cost route to edible microbial oils as well as a renewable feedstock for oleochemicals.
| Item Type: | Publication - Article |
|---|---|
| Divisions: | Plymouth Marine Laboratory > Other (PML) Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations |
| Depositing User: | Kim Hockley |
| Date made live: | 12 Mar 2019 15:00 |
| Last Modified: | 25 Apr 2020 09:59 |
| URI: | https://plymsea.ac.uk/id/eprint/8163 |
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