Spatial and temporal patterns of Pseudo-nitzschia genetic diversity in the North Pacific Ocean from the Continuous Plankton Recorder survey

Stern, RF, Moore, SK, Trainer, VL, Bill, BD, Fischer, A and Batten, SD 2018 Spatial and temporal patterns of Pseudo-nitzschia genetic diversity in the North Pacific Ocean from the Continuous Plankton Recorder survey. Marine Ecology Progress Series, 606. 7-28. https://doi.org/10.3354/meps12711

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Abstract/Summary

Several species of the marine diatom Pseudo-nitzschia can produce the neurotoxin domoic acid that is responsible for the seafood-borne illness amnesic shellfish poisoning in humans, marine wildlife mortalities and prolonged closures of fisheries resulting in economic losses to coastal communities. Since the year 2000, Pseudo-nitzschia species have been monitored in the Pacific Ocean with the Continuous Plankton Recorder (CPR). We used a combination of scanning electron microscopy with high-throughput and Sanger sequencing of CPR survey samples to compare the diversity of phytoplankton, including Pseudo-nitzschia species, from the north-eastern Pacific Ocean over 3 climatically different years: 2002, 2005 and 2008. A Pseudo-nitzschia-specific primer set targeting a partial region of the large subunit ribosomal DNA (rDNA) revealed spatially separated communities of Pseudo-nitzschia. The coastal region was dominated by a diverse array of P. fraudulenta unique sequences (operational taxonomic units), whereas the offshore region was rich in P. multiseries and contained a wide range of other Pseudo-nitzschia taxa, many not previously observed in this region. In 2008, exceptionally cold sea surface temperatures were recorded, influenced by a strong negative Pacific Decadal Oscillation signal. In that year, a more diverse assemblage of species was present in a spring open water sample, whereas P. fraudulenta was unusually rare from a coastal autumn sample. This is the first application of high-throughput genetic methods to uncover patterns of Pseudo-nitzschia genetic diversity from archival CPR samples, demonstrating the value of using CPR for plankton community analysis in rarely sampled regions of the oceans.

Item Type:	Publication - Article
Additional Keywords:	Pseudo-nitzschia, Continuous Plankton Recorder, CPR · Pacific Ocean, High-throughput sequencing, Large subunit ribosomal DNA, Genetic diversity,Harmful algae
Divisions:	Marine Biological Association of the UK > Ocean Biology
Depositing User:	Barbara Bultmann
Date made live:	18 Dec 2018 11:02
Last Modified:	09 Feb 2024 15:49
URI:	https://plymsea.ac.uk/id/eprint/8059

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