Choice of molecular barcode will affect species prevalence but not bacterial community composition

Lebret, K, Schroeder, J, Balestreri, C, Highfield, A, Cummings, DG, Smyth, TJ and Schroeder, DC 2016 Choice of molecular barcode will affect species prevalence but not bacterial community composition. Marine Genomics, 29. 39-43. https://doi.org/10.1016/j.margen.2016.09.001

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Abstract/Summary

The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.

Item Type:	Publication - Article
Subjects:	Biology Marine Sciences
Divisions:	Plymouth Marine Laboratory > National Capability categories > Western Channel Observatory
Depositing User:	Tim Smyth
Date made live:	10 Mar 2017 15:26
Last Modified:	25 Apr 2020 09:58
URI:	https://plymsea.ac.uk/id/eprint/7392

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