Tools
Tools
Bickerton, P, Sello, S, Brownlee, C, Pittman, JK and Wheeler, GL 2016 Spatial and temporal specificity of Ca2+signalling inChlamydomonas reinhardtiiin response to osmotic stress. New Phytologist. https://doi.org/10.1111/nph.14128
|
Text (full article)
nph14128.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
|
|
Other (supporting information)
nph14128-sup-0001-FigsS1-S4.pdf - Supplemental Material Available under License Creative Commons Attribution. Download (412kB) | Preview |
|
 |
Video (mp4)
Video_S1.mp4 - Supplemental Material Available under License Creative Commons Attribution. Download (83kB) |
Abstract/Summary
Ca2+-dependent signalling processes enable plants to perceive and respond to diverse environmental stressors, such as osmotic stress. A clear understanding of the role of spatiotemporal Ca2+ signalling in green algal lineages is necessary in order to understand how the Ca2+ signalling machinery has evolved in land plants. We used single-cell imaging of Ca2+-responsive fluorescent dyes in the unicellular green alga Chlamydomonas reinhardtii to examine the specificity of spatial and temporal dynamics of Ca2+ elevations in the cytosol and flagella in response to salinity and osmotic stress. We found that salt stress induced a single Ca2+ elevation that was modulated by the strength of the stimulus and originated in the apex of the cell, spreading as a fast Ca2+ wave. By contrast, hypo-osmotic stress induced a series of repetitive Ca2+ elevations in the cytosol that were spatially uniform. Hypo-osmotic stimuli also induced Ca2+ elevations in the flagella that occurred independently from those in the cytosol. Our results indicate that the requirement for Ca2+ signalling in response to osmotic stress is conserved between land plants and green algae, but the distinct spatial and temporal dynamics of osmotic Ca2+ elevations in C. reinhardtii suggest important mechanistic differences between the two lineages.
| Item Type: | Publication - Article |
|---|---|
| Additional Keywords: | calcium; Chlamydomonas reinhardtii ; flagella; green algae; osmotic stress; signalling |
| Divisions: | Marine Biological Association of the UK > Other (MBA) |
| Depositing User: | Barbara Bultmann |
| Date made live: | 01 Sep 2016 14:38 |
| Last Modified: | 25 Apr 2020 09:57 |
| URI: | https://plymsea.ac.uk/id/eprint/7151 |
Actions (login required)
 |
View Item |