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Beacham, TA, Mora Macia, V, Rooks, PA, White, DA and Ali, ST 2015 Altered Lipid Accumulation in Nannochlropsis Salina CCAP849/3 Following EMS and UV Induced Mutagenesis. Biotechnology Reports, 7. 87-94. https://doi.org/10.1016/j.btre.2015.05.007
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EMS mutagenesis paper final published version.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract/Summary
Microalgae have potential as a chemical feed stock in a range of industrial applications. Nannochloropsis salina was subject to EMS mutagenesis and the highest lipid containing cells selected using fluorescence-activated cell sorting. Assessment of growth, lipid content and fatty acid composition identified mutant strains displaying a range of altered traits including changes in the PUFA content and a total FAME increase of up to 156% that of the wild type strain. Combined with a reduction in growth this demonstrated a productivity increase of up to 76%. Following UV mutagenesis, lipid accumulation of the mutant cultures was elevated to more than 3 fold that of the wild type strain, however reduced growth rates resulted in a reduction in overall productivity. Changes observed are indicative of alterations to the regulation of the omega 6 Kennedy pathway. The importance of these variations in physiology for industrial applications such as biofuel production is discussed.
| Item Type: | Publication - Article |
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| Additional Keywords: | mutagenesis, lipids, microalgae, biofuels |
| Subjects: | Biology Ecology and Environment Marine Sciences |
| Divisions: | Plymouth Marine Laboratory > Science Areas > Microbial Processes and Biotechnology (expired) |
| Depositing User: | Sohail Ali |
| Date made live: | 30 Jun 2015 13:05 |
| Last Modified: | 25 Apr 2020 09:56 |
| URI: | https://plymsea.ac.uk/id/eprint/6419 |
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