Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification

Smolina, I, Kollias, S, Poortvliet, M, Nielsen, TG, Lindeque, PK, Castellani, C, Møller, EF, Blanco-Bercial, L and Hoarau, G 2014 Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification. Molecular Ecology Resources. n/a-n/a. https://doi.org/10.1111/1755-0998.12241

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Abstract/Summary

Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae.

Item Type:	Publication - Article
Additional Keywords:	InDels, Ion Torrent, marker design, next-generation sequencing, species delimitation, zooplankton
Subjects:	Biology Ecology and Environment Marine Sciences
Divisions:	Plymouth Marine Laboratory > Science Areas > Marine Life Support Systems (expired)
Depositing User:	Pennie Lindeque
Date made live:	10 Jun 2014 14:00
Last Modified:	25 Apr 2020 09:56
URI:	https://plymsea.ac.uk/id/eprint/6073

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