Development and validation of a molecular technique for the analysis of archived formalin-preserved phytoplankton samples permits retrospective assessment of Emiliania huxleyi communities

Ripley, SJ, Baker, AC, Miller, PI, Walne, AW and Schroeder, DC 2008 Development and validation of a molecular technique for the analysis of archived formalin-preserved phytoplankton samples permits retrospective assessment of Emiliania huxleyi communities. Journal of Microbiological Methods, 73 (2). 118-124. https://doi.org/10.1016/j.mimet.2008.02.001

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Abstract/Summary

The coccolithophores, particularly the species Emiliania huxleyi (Lohmann) Hay & Mohler, account for the bulk of global calcium carbonate production and as such play a fundamental role in global CO2 cycling and the carbonate chemistry of the oceans. To evaluate the response of this functional group to the effects of climate change, we undertook a feasibility study to determine whether a retrospective approach could be used on archived coccolithophore datasets. We demonstrate for the first time a technique for the extraction of E. huxleyi nucleic acids from archived formalin-fixed samples of the long-term Continuous Plankton Recorder. Molecular analysis of a nine year old formalin-fixed sample reveals the presence of a diverse population of E. huxleyi genotypes within a developing coccolithophore bloom. In addition, E. huxleyi sequences were amplified from a number of formalin-fixed samples, the earliest of which was collected in August 1972. This molecular assay promises the possibility of studying global variations in the distribution and genetic make-up of E. huxleyi communities over extensive periods of time. (c) 2008 Elsevier B.V. All rights reserved.

Item Type:	Publication - Article
Additional Keywords:	Continuous plankton recorder; Emiliania huxleyi; PCR
Divisions:	Marine Biological Association of the UK > Other (MBA) Plymouth Marine Laboratory > Other (PML) Sir Alister Hardy Foundation for Ocean Science > Other (SAHFOS) (expired)
Depositing User:	Miss Gemma Brice
Date made live:	26 Mar 2014 14:09
Last Modified:	06 Jun 2017 16:11
URI:	https://plymsea.ac.uk/id/eprint/5919

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