PCR from the CPR offers a historical perspective on marine population ecology.

Kirby, RR and Reid, PC 2001 PCR from the CPR offers a historical perspective on marine population ecology.. Journal of the Marine Biological Association of the United Kingdom, 81 (3). 539-540. https://doi.org/10.1017/S0025315401004192

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Abstract/Summary

The Continuous Plankton Recorder (CPR) survey has collected plankton samples from regular tracks across the world's oceans for almost 70 y. Over 299,000 spatially extensive CPR samples are archived and stored in buffered formalin. This CPR archive offers huge potential to study changes in marine communities using molecular data from a period when marine pollution, exploitation and global anthropogenic impact were much less pronounced. However, to harness the amount of data available within the CPR archive fully, it is necessary to improve techniques of larval identification, to genus and species preferably, and to obtain genetic information for historical studies of population ecology. To increase the potential of the CPR database this paper describes the first extraction, amplification by the polymerase chain reaction and utilization of a DNA sequence (mitochondrial 16S rDNA) from a CPR sample, a formalin fixed larval sandeel.

Item Type:	Publication - Article
Additional Keywords:	Analytical techniques Identification Nucleotide sequence DNA Chemotaxonomy Fish larvae Genomes Long-term records Zooplankton Polymerase chain reaction Man-induced effects
Subjects:
Divisions:	Plymouth Marine Laboratory > Other (PML) Sir Alister Hardy Foundation for Ocean Science > Other (SAHFOS) (expired)
Depositing User:	EPrints Services
Date made live:	06 Sep 2006
Last Modified:	25 Apr 2020 09:55
URI:	https://plymsea.ac.uk/id/eprint/1707

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