Electron microscopy characterization of king scallop (Pecten maximus) shells from low-voltage SEM to 3D-EBSD reconstruction

Guichaoua, L, Khurshid, B, Benchetrite, MA, Bessette, S, Stewart, B, Reznikov, N, Kröger, R and Gauvin, R 2024 Electron microscopy characterization of king scallop (Pecten maximus) shells from low-voltage SEM to 3D-EBSD reconstruction. BIO Web of Conferences, 129, 27006. 3, pp. https://doi.org/10.1051/bioconf/202412927006

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Abstract/Summary

The shell of the bivalve Pecten maximus, also called the king scallop, was previously found to be detrimentally affected by the presence of metal contamination, in particular Cu, Pb, and Zn originating from mining activities on the Isle of Man [1]. In addition to a reduction of shell thickness, scallop shells from the contaminated area exhibit a sharp break line in the mineralization within the foliated region of both the top and bottom valves. Our data suggest that these mineralization break line caused reduced fracture strength compared to pollution�free scallops, which results in increased mortality due to predation and during the process of dredging. These break lines have already been referred to as being of aragonitic prismatic structure [2]. To shed light on the possible impact of metal contaminations on the growth and strength of scallop shells and in particular on the 3D morphology and microstructure of the scallop, from contaminated and uncontaminated sites, we used characterization tools at different length scales (from cm to µm) using electron and X-ray probes to determine areas of interest in contaminated and healthy (uncontaminated) shells to finally realize crystallographic orientation map using EBSD technique and localized 3D reconstruction by focused ion beam techniques (FIB). The aim of this study was to combine these techniques to produce a full 3D EBSD map of the crystallographic orientations of the critical zones

Item Type:	Publication - Article
Additional Keywords:	Scallops, SEM-FEG, EBSD, 3D-FIB, 3D-EBSD
Subjects:	Marine Sciences Technology
Divisions:	Marine Biological Association of the UK > Ocean Biology
Depositing User:	Ms Kristina Hixon
Date made live:	15 Nov 2024 09:55
Last Modified:	15 Nov 2024 09:55
URI:	https://plymsea.ac.uk/id/eprint/10324

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