A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project

Twyford, AD, Azzopardi, F, Cunliffe, M, Uhl, R, Beasley, J, Barnes, I, Allen, H and Bell, D 2024 A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project. Wellcome Open Research, 9 (339). https://doi.org/10.12688/wellcomeopenres.21143.1

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Abstract/Summary

Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development and implementation of DNA barcoding for the Darwin Tree of Life Project (DToL), which aims to sequence and assemble high quality reference genomes for all eukaryotic species in Britain and Ireland. We present a standardised framework for DNA barcode sequencing and data interpretation that is then adapted for diverse organismal groups. DNA barcoding data from over 12,000 DToL specimens has identified up to 20% of samples requiring additional verification, with 2% of seed plants and 3.5% of animal specimens subsequently having their names changed. We also make recommendations for future developments using new sequencing approaches and streamlined bioinformatic approaches

Item Type:	Publication - Article
Additional Keywords:	DNA barcoding, species identification, taxonomy, Britain and Ireland, biodiversity
Subjects:	Marine Sciences
Divisions:	Marine Biological Association of the UK > Other (MBA)
Depositing User:	Ms Kristina Hixon
Date made live:	15 Nov 2024 09:55
Last Modified:	15 Nov 2024 09:55
URI:	https://plymsea.ac.uk/id/eprint/10323

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