Colonial choanoflagellate isolated from Mono Lake harbors a microbiome

Hake, KH; West, PT; McDonald, K; Laundon, D; Feng, C; Burkhardt, P; Richter, D; Banfield, JF; King, N. 2021 Colonial choanoflagellate isolated from Mono Lake harbors a microbiome. Pre-print - bioRxiv.

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Choanoflagellates offer key insights into bacterial influences on the origin and early evolution of animals. Here we report the isolation and characterization of a new colonial choanoflagellate species, Barroeca monosierra, that, unlike previously characterized species, harbors a stable microbiome. B. monosierra was isolated from Mono Lake, California and forms large spherical colonies that are more than an order of magnitude larger than those formed by the closely related Salpingoeca rosetta. By designing fluorescence in situ hybridization probes from metagenomic sequences, we found that B. monosierra colonies are colonized by members of the halotolerant and closely related Saccharospirillaceae and Oceanospirillaceae, as well as purple sulfur bacteria (Ectothiorhodospiraceae) and non-sulfur Rhodobacteraceae. This relatively simple microbiome in a close relative of animals presents a new experimental model for investigating the evolution of stable interactions among eukaryotes and bacteria.

Item Type: Publication - Article
Subjects: Marine Sciences
Divisions: Marine Biological Association of the UK > Mechanisms underlying biogeochemical and ecological processes > Cell and Molecular Programme
Depositing User: Emily Smart
Date made live: 08 Oct 2021 14:57
Last Modified: 08 Oct 2021 14:57

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