Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean

Mary, I, Tarran, GA, Warwick, PE, Terry, MJ, Scanlan, DJ, Burkill, PH and Zubkov, MV 2008 Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean. FEMS Microbiology Ecology, 63 (1). 36-45.

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35S-Methionine and 3H-leucine bioassay tracer experiments were conducted on two meridional transatlantic cruises to assess whether dominant planktonic microorganisms use visible sunlight to enhance uptake of these organic molecules at ambient concentrations. The two numerically dominant groups of oceanic bacterioplankton were Prochlorococcus cyanobacteria and bacteria with low nucleic acid (LNA) content, comprising 60% SAR11-related cells. The results of flow cytometric sorting of labelled bacterioplankton cells showed that when incubated in the light, Prochlorococcus and LNA bacteria increased their uptake of amino acids on average by 50% and 23%, respectively, compared with those incubated in the dark. Amino acid uptake of Synechococcus cyanobacteria was also enhanced by visible light, but bacteria with high nucleic acid content showed no light stimulation. Additionally, differential uptake of the two amino acids by the Prochlorococcus and LNA cells was observed. The populations of these two types of cells on average completely accounted for the determined 22% light enhancement of amino acid uptake by the total bacterioplankton community, suggesting a plausible way of harnessing light energy for selectively transporting scarce nutrients that could explain the numerical dominance of these groups in situ.

Item Type: Publication - Article
Additional Information. Not used in RCUK Gateway to Research.: non-CPR
Additional Keywords: marine phytoplankton;microbial photoheterotrophy;amino acid transport;flow cytometric sorting;dual tracer labelling
Divisions: Plymouth Marine Laboratory > Other (PML)
Sir Alister Hardy Foundation for Ocean Science > Other (SAHFOS) (expired)
Depositing User: Miss Gemma Brice
Date made live: 26 Mar 2014 14:09
Last Modified: 25 Apr 2020 09:56

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