Vitamin B12 bioaccumulation in Chlorella vulgaris grown on food waste-derived anaerobic digestate

Papadopoulos, KP, Fernandes de Souza, M, Archer, L, Illanes, ACZ, Harrison, EL, Taylor, F, Davey, MP, Gallardo, DA, Komakech, AJ and Radmehr, S 2023 Vitamin B12 bioaccumulation in Chlorella vulgaris grown on food waste-derived anaerobic digestate. Algal Research, 75.

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Anaerobic digestion plays a pivotal role in the modern circular economy, as it offers a sustainable solution for converting organic waste into biogas (methane). It also results in a nutrient-rich liquid stream, referred to as digestate. This digestate is extensively applied to agricultural land as fertilizer due to its high macronutrient (N, P) content, but the bioactive micronutrients it contains and their significance for downstream applications remain largely unknown. Here, we investigate whether digestate generated from a vitamin B12-deficient substrate (fruit and vegetable waste) can be enriched in this vitamin through anaerobic digestion, and explore the capability of the microalga Chlorella vulgaris to grow in this medium and bioaccumulate B12. Our findings uncover, for the first time, that substantial amounts of B12 are synthesized during anaerobic digestion, and that C. vulgaris can effectively be enriched with this vitamin when grown in the digestate (10.6 μg Β12·g−1 dry weight). Additionally, we identified that pH-induced ammonia toxicity was the main inhibitor when growing microalgae in the digestate, which allowed us to significantly enhance productivity at lab- and pilot-scale through pH control. The case of B12 synthesis in digestate and accumulation in microalgae highlights the potential for enhancing the value of these waste streams through the identification and utilization of bioactive compounds.

Item Type: Publication - Article
Additional Keywords: cobalamin, algae, bioremediation, photobioreactor, aquaculture
Subjects: Aquaculture
Marine Sciences
Divisions: Marine Biological Association of the UK > Other (MBA)
Depositing User: Ms Kristina Hixon
Date made live: 21 Dec 2023 15:21
Last Modified: 09 Feb 2024 16:56

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