Seasonal monitoring of lipid degradation processes in the western English Channel links bacterial 10S-DOX enzyme activity to free fatty acid production by phytoplankton

Rontani, JF; Smik, L; Vaultier, F; Widdicombe, CE; Belt, ST. 2021 Seasonal monitoring of lipid degradation processes in the western English Channel links bacterial 10S-DOX enzyme activity to free fatty acid production by phytoplankton. Marine Chemistry, 230. 103928. https://doi.org/10.1016/j.marchem.2021.103928 (Unpublished)

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Official URL: http://dx.doi.org/10.1016/j.marchem.2021.103928

Abstract/Summary

. In a few recent studies, the action of a bacterial dioxygenase (10S-DOX) on palmitoleic acid was observed within some polar and estuarine settings. To add further mechanistic information regarding the action of this enzyme in marine settings, we measured a range of lipids (sterols, fatty acids and the chlorophyll phytyl side chain) and their biotic and abiotic degradation products in water samples collected in 2018 from two depths (5 m and 25 m) at the temperate oceanographic time series site L4, located in the western English Channel. Lipid distributions indicated a dominance of diatoms and copepods during the spring bloom, while a peak in dinoflagellate activity was evident in samples collected from late summer/autumn, both outcomes being consistent with taxonomic data reported previously for the same sampling site and interval. Monitoring of lipid oxidation products characteristic of different degradation pathways showed a relatively weak effect of photo- and autoxidation processes, with these acting mainly on the more reactive lipids (i.e. chlorophyll and polyunsaturated fatty acids). In contrast, monitoring of biotic degradation processes revealed significant quantities of 10S-hydroxyhexadec-8(E)-enoic acid in samples collected at the end of April (reaching 40% of the residual parent palmitoleic acid), attributed to the involvement of bacterial 10-dioxygenase (10S-DOX) activity during the spring bloom. We propose that this enzyme could be utilised by bacteria to detoxify free fatty acids released by wounded diatoms in the presence of copepods

Item Type: Publication - Article
Additional Keywords: Biotic and abiotic degradation; 10S-DOX enzymatic activity; Bacteria; Wounded diatoms.
Divisions: Plymouth Marine Laboratory > National Capability categories > Single Centre NC - CLASS
Plymouth Marine Laboratory > Science Areas > Marine Ecology and Biodiversity
Depositing User: S Hawkins
Date made live: 10 Mar 2022 11:34
Last Modified: 10 Mar 2022 11:34
URI: http://plymsea.ac.uk/id/eprint/9622

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