Effect of FMRFamide on voltage-dependent currents in identified centrifugal neurons of the optic lobe of the cuttlefish, Sepia officinalis

Chrachri, A. 2020 Effect of FMRFamide on voltage-dependent currents in identified centrifugal neurons of the optic lobe of the cuttlefish, Sepia officinalis. bioRxiv. https://doi.org/10.1101/2020.09.29.318691

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Official URL: https://www.biorxiv.org/content/10.1101/2020.09.29...

Abstract/Summary

Whole-cell patch-clamp recordings from identified centrifugal neurons of the optic lobe in a slice preparation allowed the characterization of five voltage-dependent currents; two outward and three inward currents. The outward currents were; the 4-aminopyridine-sensitive transient potassium or A-current (IA), the TEA-sensitive sustained current or delayed rectifier (IK). The inward currents were; the tetrodotoxin sensitive transient current or sodium current (INa). The second is the cobalt- and cadmium-sensitive sustained current which is enhanced by barium and blocked by the dihydropyridine antagonist, nifedipine suggesting that it could be the L-type calcium current (ICaL). Finally, another transient inward current, also carried by calcium, but unlike the L-type, this current is activated at more negative potentials and resembles the low-voltage-activated or T-type calcium current (ICaT) of other preparations. Application of the neuropeptide FMRFamide caused a significant attenuation to the peak amplitude of both sodium and sustained calcium currents without any apparent effect on the transient calcium current. Furthermore, FMRFamide also caused a reduction of both outward currents in these centrifugal neurons. The fact that FMRFamide reduced the magnitude of four of five characterized currents could suggest that this neuropeptide may act as a strong inhibitory agent on these neurons.

Item Type: Publication - Article
Additional Keywords: cephalopod, voltage-clamp, potassium current, calcium currents, sodium current, FMRFamide.
Subjects: Marine Sciences
Divisions: Marine Biological Association of the UK > Mechanisms underlying biogeochemical and ecological processes > Phytoplankton Cell and Membrane Physiology
Depositing User: Emily Smart
Date made live: 08 Oct 2021 14:58
Last Modified: 08 Oct 2021 14:58
URI: http://plymsea.ac.uk/id/eprint/9429

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