Unravelling the developmental and functional significance of an ancient Argonaute duplication

Fridrich, A; Modepalli, V; Lewandowska, M; Aharoni, R; Moran, Y. 2020 Unravelling the developmental and functional significance of an ancient Argonaute duplication. Nature Communications, 11, 6187. https://doi.org/10.1038/s41467-020-20003-8

[img]
Preview
Text
109 Unravelling the developmental and functional significance of an ancient Argonaute duplication.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
Official URL: https://www.nature.com/articles/s41467-020-20003-8...

Abstract/Summary

MicroRNAs (miRNAs) base-pair to messenger RNA targets and guide Argonaute proteins to mediate their silencing. This target regulation is considered crucial for animal physiology and development. However, this notion is based exclusively on studies in bilaterians, which comprise almost all lab model animals. To fill this phylogenetic gap, we characterize the functions of two Argonaute paralogs in the sea anemone Nematostella vectensis of the phylum Cnidaria, which is separated from bilaterians by ~600 million years. Using genetic manipulations, Argonaute-immunoprecipitations and high-throughput sequencing, we provide experimental evidence for the developmental importance of miRNAs in a non-bilaterian animal. Additionally, we uncover unexpected differential distribution of distinct miRNAs between the two Argonautes and the ability of one of them to load additional types of small RNAs. This enables us to postulate a novel model for evolution of miRNA precursors in sea anemones and their relatives, revealing alternative trajectories for metazoan miRNA evolution.

Item Type: Publication - Article
Additional Keywords: miRNAs, molecular evolution
Subjects: Marine Sciences
Divisions: Marine Biological Association of the UK > Mechanisms underlying biogeochemical and ecological processes > Cell and Molecular Programme
Depositing User: Emily Smart
Date made live: 28 Sep 2021 14:46
Last Modified: 28 Sep 2021 14:46
URI: http://plymsea.ac.uk/id/eprint/9389

Actions (login required)

View Item View Item