Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Highfield, A; Stern, R; Wheeler, G; Dupont, CL; Edgcomb, V; Einarsson, E; Elustondo, PA; Federici, F; Freire-Beneitez, V; Freyria et al, NJ. 2020 Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17. 481-494. https://doi.org/10.1038/s41592-020-0796-x

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Abstract/Summary

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Item Type: Publication - Article
Subjects: Marine Sciences
Divisions: Marine Biological Association of the UK > Mechanisms underlying biogeochemical and ecological processes > Phytoplankton Cell and Membrane Physiology
Depositing User: Emily Smart
Date made live: 28 Sep 2021 13:02
Last Modified: 28 Sep 2021 13:02
URI: http://plymsea.ac.uk/id/eprint/9355

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