Advances in the Detection of Toxic Algae Using Electrochemical Biosensors

Medlin, Linda K.; Gamella, Maria; Mengs, G; Serrafin, V; Campuzano, S; Pingarrón, J. 2020 Advances in the Detection of Toxic Algae Using Electrochemical Biosensors. Biosensors, 10. https://doi.org/10.3390/bios10120207

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Abstract/Summary

Harmful algal blooms (HABs) are more frequent as climate changes and tropical toxic species move northward, especially along the Iberian Peninsula, a rich aquaculture area. Monitoring programs, detecting the presence of toxic algae before they bloom, are of paramount importance to protect ecosystems, aquaculture, human health and local economies. Rapid, reliable species identification methods using molecular barcodes coupled to biosensor detection tools have received increasing attention as an alternative to the legally required but impractical microscopic counting-based techniques. Our electrochemical detection system has improved, moving from conventional sandwich hybridization protocols using di�erent redox mediators and signal probes with di�erent labels to a novel strategy involving the recognition of RNA heteroduplexes by antibodies further labelled with bacterial antibody binding proteins conjugated with multiple enzyme molecules. Each change has increased sensitivity. A 150-fold signal increase has been produced with our newest protocol using magnetic microbeads (MBs) and amperometric detection at screen-printed carbon electrodes (SPCEs) to detect the target RNA of toxic species. We can detect as few as 10 cells L

Item Type: Publication - Article
Additional Keywords: toxic algae; biosensors; barcodes; early warning system
Subjects: Biology
Botany
Management
Marine Sciences
Divisions: Marine Biological Association of the UK > Ecosystems and Environmental Change > Marine Biodiversity and Climate Change
Depositing User: Prof Linda Medlin
Date made live: 09 Feb 2021 10:32
Last Modified: 09 Feb 2021 10:32
URI: http://plymsea.ac.uk/id/eprint/9107

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