Anti-oxidative hormetic effects of cellular autophagy induced by nutrient deprivation in a molluscan animal model

Moore, MN; Shaw, JP; Pascoe, CK; Beesley, A; Viarengo, A; Lowe, DM. 2020 Anti-oxidative hormetic effects of cellular autophagy induced by nutrient deprivation in a molluscan animal model. Marine Environmental Research, 156. 104903. https://doi.org/10.1016/j.marenvres.2020.104903

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Official URL: http://dx.doi.org/10.1016/j.marenvres.2020.104903

Abstract/Summary

This investigation using a molluscan animal model tested the hypothesis that experimentally induced lysosomal autophagy protects against oxidative cell injury. Induction of augmented lysosomal autophagy has previously been implicated in this protective process. Four treatment groups of blue mussels (Mytilus galloprovincialis) were used: Group 1 (fed - control), Group 2 (fasted), Group 3 (copper þfed) and Group 4 (copper þfasted). Groups 2 and 4 were fasted in order to trigger autophagy; and samples of hepatopancreas (liver analogue or digestive gland) from all 4 groups were taken at 3, 6 and 15 days. Treatment with copper provided a positive reference for oxidative stress: Groups 3 and 4 were treated with copper (10 μg Cu2þ/animal/day) for three days only. Oxidative damage and cellular injury in hepatopancreatic digestive cells was found to decrease in Group 2 (fasted) compared to Group 1 (fed - control). Group 3 (fed þcopper) showed clear evidence of oxidative stress and cell injury, as well as induction of antioxidant activities. Group 4 (copper þfasted) had a reduced uptake of copper and toxicity of copper was also reduced, compared with Group 3. It was concluded that augmented autophagy had a hormetic cytoprotective anti-oxidant effect. 1

Item Type: Publication - Article
Additional Keywords: Autophagy Copper Cytoprotection Fasting Hepatopancreas Digestive gland Hormesis Lipofuscin Lysosomes Mussel Oxidative cell injury
Divisions: Plymouth Marine Laboratory > Other (PML)
Depositing User: S Hawkins
Date made live: 10 Jun 2020 09:13
Last Modified: 10 Jun 2020 09:13
URI: http://plymsea.ac.uk/id/eprint/8972

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