Co-occurrence of Fe and P stress in natural populations of the marine diazotroph <i>Trichodesmium</i>

Held, NA; Webb, EA; McIlvin, MM; Hutchins, DA; Cohen, NR; Moran, DM.; Kunde, K; Lohan, MC; Mahaffey, C; Woodward, EMS; Saito, MA. 2020 Co-occurrence of Fe and P stress in natural populations of the marine diazotroph <i>Trichodesmium</i>. Biogeosciences, 17 (9). 2537-2551. https://doi.org/10.5194/bg-17-2537-2020

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Official URL: http://dx.doi.org/10.5194/bg-17-2537-2020

Abstract/Summary

Trichodesmiumisagloballyimportantmarinemicrobethatprovidesfixednitrogen(N)tootherwiseN-limited ecosystems.Innature,nitrogenfixationislikelyregulatedby iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses using established protein biomarkers for nutrient stress, we found that iron–phosphate co-stress is the norm rather than the exception for Trichodesmium colonies in the North AtlanticOcean.Counterintuitively,thenitrogenaseenzymewas more abundant under co-stress as opposed to single nutrient stress. This is consistent with the idea that Trichodesmium has a specific physiological state during nutrient co-stress. Organic nitrogen uptake was observed and occurred simultaneously with nitrogen fixation. The quantification of the phosphate ABC transporter PstA combined with a cellular model of nutrient uptake suggested that Trichodesmium is generally confronted by the biophysical limits of membrane space and diffusion rates for iron and phosphate acquisition in the field. Colony formation may benefit nutrient acquisition from particulate and organic sources, alleviating these pressures. The results highlight that to predict the behavior of Trichodesmium, both Fe and P stress must be evaluated simultaneously.

Item Type: Publication - Article
Additional Information. Not used in RCUK Gateway to Research.: Correspondence:Mak A. Saito (msaito@whoi.edu)
Divisions: Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations
Depositing User: S Hawkins
Date made live: 03 Jun 2020 09:16
Last Modified: 03 Jun 2020 09:16
URI: http://plymsea.ac.uk/id/eprint/8954

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