Engineering The Unicellular Alga Phaeodactylum tricornutum For High-Value Plant Triterpenoid Production

D'Adamo, S; Schiano di Visconte, G; Lowe, G; Szaub-Newton, J; Beacham, TA; Landels, A; Allen, MJ; Spicer, A; Matthijs, M. 2018 Engineering The Unicellular Alga Phaeodactylum tricornutum For High-Value Plant Triterpenoid Production. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12948

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Abstract/Summary

Plant triterpenoids constitute a diverse class of organic compounds that play a major role in development, plant defense and environmental interaction. Several triterpenes have demonstrated potential as pharmaceuticals. One example is betulin, which has shown promise as a pharmaceutical precursor for the treatment of certain cancers and HIV. Major challenges for triterpenoid commercialization include their low production levels and their cost‐effective purification from the complex mixtures present in their natural hosts. Therefore, attempts to produce these compounds in industrially relevant microbial systems such as bacteria and yeasts have attracted great interest. Here we report the production of the triterpenes betulin and its precursor lupeol in the photosynthetic diatom Phaeodactylum tricornutum, a unicellular eukaryotic alga. This was achieved by introducing three plant enzymes in the microalga: a Lotus japonicus oxidosqualene cyclase and a Medicago truncatula cytochrome P450 along with its native reductase. The introduction of the L. japonicus oxidosqualene cyclase perturbed the mRNA expression levels of the native mevalonate and sterol biosynthesis pathway. The best performing strains were selected and grown in a 550L pilot scale photobioreactor facility. To our knowledge, this is the most extensive pathway engineering undertaken in a diatom and the first time that a sapogenin has been artificially produced in a microalga, demonstrating the feasibility of the photo‐bio‐production of more complex high‐value, metabolites in microalgae.

Item Type: Publication - Article
Subjects: Technology
Divisions: Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations
Depositing User: Mike Allen
Date made live: 18 Jun 2018 15:52
Last Modified: 25 Apr 2020 09:59
URI: http://plymsea.ac.uk/id/eprint/7934

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