An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment

Teague, J; Scott, T; Sharma, S; Graham, G; Allen, MJ. 2017 An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. Journal of Marine Science and Engineering, 5 (2). 22. https://doi.org/10.3390/jmse5020022

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Official URL: http://www.mdpi.com/2077-1312/5/2/22

Abstract/Summary

The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality.

Item Type: Publication - Article
Subjects: Ecology and Environment
Marine Sciences
Oceanography
Technology
Divisions: Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations
Plymouth Marine Laboratory > Science Areas > Microbial Processes and Biotechnology (expired)
Depositing User: Mike Allen
Date made live: 22 Aug 2018 13:39
Last Modified: 25 Apr 2020 09:58
URI: http://plymsea.ac.uk/id/eprint/7453

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