Occurrence of chlorophyll allomers during virus‐induced mortality and population decline in the ubiquitous picoeukaryote Ostreococcus tauri

Steele, DJ; Kimmance, SA; Franklin, DJ; Airs, RL. 2017 Occurrence of chlorophyll allomers during virus‐induced mortality and population decline in the ubiquitous picoeukaryote Ostreococcus tauri. Environmental Microbiology. 10.1111/1462-2920.13980

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Abstract/Summary

During viral infection and growth limitation of the picoeukaryote Ostreococcus tauri, we examined the relationship between membrane permeability, oxidative stress and chlorophyll allomers (oxidation products). Chlorophyll allomers were measured in batch-cultures of O. tauri in parallel with maximum quantum efficiency of photosystem II photochemistry (Fv/Fm), carotenoids, and reactive oxygen species and membrane permeability using fluorescent probes (CM-H2DCFDA and SYTOX-Green). Viral infection led to mass cell lysis of the O. tauri cells within 48 h. The concentration of hydroxychlorophyll a peaked with a 16-fold increase (relative to chl-a) just after the major lysis event. In contrast, cell death due to growth limitation resulted in a 2-fold increase in allomer production, relative to chlorophyll a. Two allomers were detected solely in association with O. tauri debris after viral lysis, and unlike other allomers were not observed before viral lysis, or during cell death due to growth limitation. Conversely, the component chl-aP276 was found in the highest concentrations relative to chl-a, in exponentially growing O. tauri. The components described have potential as indicators of mode of phytoplankton cell mortality.

Item Type: Publication - Article
Additional Information. Not used in RCUK Gateway to Research.: "This is the peer reviewed version of the following article: 'Steele, DJ; Kimmance, SA; Franklin, DJ; Airs, RL. 2017 Occurrence of chlorophyll allomers during virus‐induced mortality and population decline in the ubiquitous picoeukaryote Ostreococcus tauri. Environmental Microbiology' which has been published in final form at 10.1111/1462-2920.13980. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
Subjects: Ecology and Environment
Marine Sciences
Oceanography
Divisions: Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations
Depositing User: Dr Susan Kimmance
Date made live: 10 Feb 2018 11:13
Last Modified: 13 Mar 2018 15:32
URI: http://plymsea.ac.uk/id/eprint/7600

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