Microarray (phylochip) analysis of freshwater pathogens at several sites along the Northern German coast transecting both estuarine and freshwaters

Baudart, J; Guillebault, D; Meilke, E; Meyer, T; Tandon, N; Fischer, S; Weigel, W; Medlin, LK. 2017 Microarray (phylochip) analysis of freshwater pathogens at several sites along the Northern German coast transecting both estuarine and freshwaters. Applied Microbiology and Biotechnology, 101 (2). 871-886. 10.1007/s00253-016-7937-2

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Abstract/Summary

Monitoring the quality of drinking water is an important issue for public health. Two of the main objectives of the European Project µAqua were (i) the development of specific probes to detect and quantify pathogens in drinking water and (ii) the design of standardized sampling programs of water from different sources in Europe in order to obtain sufficient material for downstream analysis. Our phylochip contains barcodes that specifically identify freshwater pathogens for enabling the detection of organisms that can be risks for human health. Monitoring for organisms with molecular tools are rapid, more accurate and more reliable than traditional methods. Rapid detection means that mitigation strategies come into play faster with less harm to the community and to humans. Samples were collected from several waters in France, Germany, Ireland, Italy and Turkey over two years. We present microarray results for the presence of freshwater pathogens from brackish and freshwater sites in northern Germany, and cyanobacterial cell numbers inferred from these sites. In a companion study from the same samples, cyanobacterial toxins were analysed using two methods and those sites with highest toxin values also had highest cell numbers as inferred from this microarray study.

Item Type: Publication - Article
Additional Information. Not used in RCUK Gateway to Research.: An erratum to this article is available at http://dx.doi.org/10.1007/s00253-016-8040-4
Additional Keywords: phylochips, microarray, freshwater pathogens, cyanobacteria, molecular barcodes
Subjects: Botany
Divisions: Marine Biological Association of the UK > Ecosystems and Environmental Change > Marine Biodiversity and Climate Change
Depositing User: Dr Linda Medlin
Date made live: 20 Feb 2017 15:47
Last Modified: 06 Jun 2017 16:17
URI: http://plymsea.ac.uk/id/eprint/7317

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