Spatial and temporal specificity of Ca2+signalling inChlamydomonas reinhardtiiin response to osmotic stress

Bickerton, P; Sello, S; Brownlee, C; Pittman, JK; Wheeler, GL. 2016 Spatial and temporal specificity of Ca2+signalling inChlamydomonas reinhardtiiin response to osmotic stress. New Phytologist. 10.1111/nph.14128

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Official URL: http://dx.doi.org/10.1111/nph.14128

Abstract/Summary

Ca2+-dependent signalling processes enable plants to perceive and respond to diverse environmental stressors, such as osmotic stress. A clear understanding of the role of spatiotemporal Ca2+ signalling in green algal lineages is necessary in order to understand how the Ca2+ signalling machinery has evolved in land plants. We used single-cell imaging of Ca2+-responsive fluorescent dyes in the unicellular green alga Chlamydomonas reinhardtii to examine the specificity of spatial and temporal dynamics of Ca2+ elevations in the cytosol and flagella in response to salinity and osmotic stress. We found that salt stress induced a single Ca2+ elevation that was modulated by the strength of the stimulus and originated in the apex of the cell, spreading as a fast Ca2+ wave. By contrast, hypo-osmotic stress induced a series of repetitive Ca2+ elevations in the cytosol that were spatially uniform. Hypo-osmotic stimuli also induced Ca2+ elevations in the flagella that occurred independently from those in the cytosol. Our results indicate that the requirement for Ca2+ signalling in response to osmotic stress is conserved between land plants and green algae, but the distinct spatial and temporal dynamics of osmotic Ca2+ elevations in C. reinhardtii suggest important mechanistic differences between the two lineages.

Item Type: Publication - Article
Additional Keywords: calcium; Chlamydomonas reinhardtii ; flagella; green algae; osmotic stress; signalling
Divisions: Marine Biological Association of the UK > Other (MBA)
Depositing User: Barbara Bultmann
Date made live: 01 Sep 2016 14:38
Last Modified: 06 Jun 2017 16:16
URI: http://plymsea.ac.uk/id/eprint/7151

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