The application of randomly amplified polymorphic DNA (RAPD's) to the study of Lasaea rubra (Montagu).

Tyler-Walters, H; Hawkins, AR. 1995 The application of randomly amplified polymorphic DNA (RAPD's) to the study of Lasaea rubra (Montagu).. Journal of the Marine Biological Association of the United Kingdom, 75 (3). 563-569. /10.1017/S0025315400039011

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Official URL: http://journals.cambridge.org/article_S00253154000...

Abstract/Summary

This paper describes the random amplification of polymorphic DNA markers (RAPDs) in Lasaea rubra (Erycinidae: Bivalvia). Present evidence suggests that L. rubra is an asexual species; however, the exact mode of clonal reproduction in this species is still a matter of debate. In this preliminary study, four of the primers used generated polymorphic RAPDs. One primer was able to distinguish between individuals from the same or different crevice population. This same primer also resolved a single band difference between otherwise identical RAPD patterns of a parent and its offspring. No familial differences have been detected in several previous studies using allozyme electrophoresis. This paper suggests that many polymorphic markers could be obtained with this species using the RAPD technique. Population genetic analysis of L. rubra has long been hampered by a dearth of polymorphic markers due to its small size. These findings suggest that this technique has the potential to further the study of population genetics in this asexual species.

Item Type: Publication - Article
Subjects: Marine Sciences
Divisions: Marine Biological Association of the UK > Knowledge Exchange > Evidence
Depositing User: Dr Harvey Tyler-Walters
Date made live: 16 May 2016 10:57
Last Modified: 06 Jun 2017 16:16
URI: http://plymsea.ac.uk/id/eprint/7014

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