Atmospheric pressure chemical ionisation liquid chromatography/mass spectrometry of bacteriochlorophylls from Chlorobiaceae: characteristic fragmentations

Airs, RL; Keely, BJ. 2002 Atmospheric pressure chemical ionisation liquid chromatography/mass spectrometry of bacteriochlorophylls from Chlorobiaceae: characteristic fragmentations. Rapid Communications in Mass Spectrometry, 16 (5). 453-461. 10.1002/rcm.598

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Official URL: http://dx.doi.org/10.1002/rcm.598

Abstract/Summary

Atmospheric pressure chemical ionisation liquid chromatography/mass spectrometry/mass spectrometry (APCI-LC/MS/MS) has been applied to the study of bacteriochlorophylls c, d, and e of phototrophic prokaryotes. Cultures of Chlorobiaceae containing bacteriochlorophyll c, d or e were examined using a high-resolution high-performance liquid chromatography (HPLC) method and APCI-LC/MS/MS employing post-column addition of formic acid. The results reveal complex distributions of bacteriochlorophyll homologues, with some closely eluting species giving isobaric protonated molecules. On-line LC/MS/MS studies reveal characteristic fragment ions for bacteriochlorophylls c, d, and e. Fragmentations involving loss of the extended alkyl substituents that are unique to bacteriochlorophylls c, d and e and their derivatives have been rationalised by studying the phaeophorbides and the results applied to the direct study of the bacteriochlorophylls.

Item Type: Publication - Article
Subjects: Chemistry
Marine Sciences
Divisions: Plymouth Marine Laboratory > Science Areas > Cycling in the Sunlit Ocean
Depositing User: Ruth Airs
Date made live: 16 Feb 2016 11:59
Last Modified: 06 Jun 2017 16:15
URI: http://plymsea.ac.uk/id/eprint/6796

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