Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina

Beacham, TA; Ali, ST. 2016 Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina. Algal Research, 14. 65-71. /10.1016/j.algal.2016.01.005

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Here we report recombinant expression and activity of the Saccharomyces cerevisiae type 2 diacylglycerol acyltransferase DGA1 functioning in parallel with the native Nannochloropsis salina genes. Expression of DGA1 shifted the chain length distribution of fatty acids produced and reflected an oleoyl-CoA substrate preference. Effect on the total FAME content was moderate and elevated by a maximum of 38%. Expression of the DGA1 transgene varied throughout the culture life cycle and evidence of growth dependent environmental silencing of the transgene was observed. This is to our knowledge the first example of silencing and subsequent resetting in a transgenic microalga. Results from this study add valuable insights into the efficacy of algal genetic engineering and use of these microorganisms as bio-platforms for chemical manufacture.

Item Type: Publication - Article
Additional Keywords: Nannochloropsis; Microalgae; Lipid; Biofuels; Silencing; Chromatin
Subjects: Ecology and Environment
Marine Sciences
Divisions: Plymouth Marine Laboratory > Science Areas > Marine Biochemistry and Observations
Depositing User: Dr Tracey Beacham
Date made live: 10 Jun 2016 10:42
Last Modified: 06 Jun 2017 16:14

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