Altered Lipid Accumulation in Nannochlropsis Salina CCAP849/3 Following EMS and UV Induced Mutagenesis

Beacham, TA; Mora Macia, V; Rooks, PA; White, DA; Ali, ST. 2015 Altered Lipid Accumulation in Nannochlropsis Salina CCAP849/3 Following EMS and UV Induced Mutagenesis. Biotechnology Reports, 7. 87-94. 10.1016/j.btre.2015.05.007

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Abstract/Summary

Microalgae have potential as a chemical feed stock in a range of industrial applications. Nannochloropsis salina was subject to EMS mutagenesis and the highest lipid containing cells selected using fluorescence-activated cell sorting. Assessment of growth, lipid content and fatty acid composition identified mutant strains displaying a range of altered traits including changes in the PUFA content and a total FAME increase of up to 156% that of the wild type strain. Combined with a reduction in growth this demonstrated a productivity increase of up to 76%. Following UV mutagenesis, lipid accumulation of the mutant cultures was elevated to more than 3 fold that of the wild type strain, however reduced growth rates resulted in a reduction in overall productivity. Changes observed are indicative of alterations to the regulation of the omega 6 Kennedy pathway. The importance of these variations in physiology for industrial applications such as biofuel production is discussed.

Item Type: Publication - Article
Additional Keywords: mutagenesis, lipids, microalgae, biofuels
Subjects: Biology
Ecology and Environment
Marine Sciences
Divisions: Plymouth Marine Laboratory > Science Areas > Microbial Processes and Biotechnology
Depositing User: Sohail Ali
Date made live: 30 Jun 2015 13:05
Last Modified: 06 Jun 2017 16:13
URI: http://plymsea.ac.uk/id/eprint/6419

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